Abstract-Label‐free bacterial colony detection and viability assessment by continuous‐wave terahertz transmission imaging

Xiang Yang,   Jia Shi,  Yuye Wang,   Ke Yang,   Xiang Zhao,   Guiyu Wang,   Degang Xu,   Yunxia Wang,  Jianquan Yao,   Weiling Fu,

https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700386

Timely and accurate bacterial detection is critical for various health and safety applications, which promotes the continuous development of versatile optical sensors for bacterial investigations. Here, we report a new strategy for bacterial colony sensing using terahertz (THz) imaging with minimal assay procedures. The proposed method utilizes the acute sensitivity of THz wave to the changes in the water content and cellular structures. Single bacterial colonies of four bacterial species were directly distinguished using THz imaging by utilizing their differences in THz absorption. In addition, the distribution of mixed bacterial samples has been demonstrated by THz imaging, which demonstrated that the target bacterium could be easily recognized. Furthermore, we investigated the differentiation of bacterial viability, which indicated that bacteria under different living states could be distinguished by THz imaging because of their different hydration levels and cellular structures. Our results suggest that THz imaging has the potential to be used for mixed bacterial sample detection and bacterial viability assessment in a label‐free and nondestructive manner.

Abstract-Cell viability and hydration assay based on metamaterial-enhanced terahertz spectroscopy

Yu Liu,  Mingjie Tang,  Liangping Xia,  Wenjing Yu,  Jia Peng, Yang Zhang,  Marc Lamy de la Chapelle,  Ke Yang,  Hong-Liang Cui,  Weiling Fu

http://pubs.rsc.org/en/content/articlelanding/2017/ra/c7ra09609g#!divAbstract

As a fast-growing technology, terahertz time-domain spectroscopy (THz-TDS) is becoming increasingly pervasive in biological applications, targeting a range of biomaterials from biomolecules to tissues. However, THz-TDS studies at the cellular level are quite limited. Thus, a study to analyze the living state and hydration state of a tumor cell in a label-free manner is carried out and reported here. Combined with a specially designed THz metamaterial, a tumor cell monolayer was detected continuously over a period of time. In addition, in order to explore the possible impact of the metamaterial on tumor cells, the secretion of IL-6, IL-8, GM-CSF and GROα of cell supernatants was detected. The results demonstrated that the technology could characterize the living state by monitoring the extracellular water and investigate the hydration state inside a tumor cell in real time, showing great application potential for the label-free detection of normal cells and tumor cells of diverse malignant degree.