Yu Liu, Mingjie Tang, Liangping Xia, Wenjing Yu, Jia Peng, Yang Zhang, Marc Lamy de la Chapelle, Ke Yang, Hong-Liang Cui, Weiling Fu
http://pubs.rsc.org/en/content/articlelanding/2017/ra/c7ra09609g#!divAbstract
As a fast-growing technology, terahertz time-domain spectroscopy (THz-TDS) is becoming increasingly pervasive in biological applications, targeting a range of biomaterials from biomolecules to tissues. However, THz-TDS studies at the cellular level are quite limited. Thus, a study to analyze the living state and hydration state of a tumor cell in a label-free manner is carried out and reported here. Combined with a specially designed THz metamaterial, a tumor cell monolayer was detected continuously over a period of time. In addition, in order to explore the possible impact of the metamaterial on tumor cells, the secretion of IL-6, IL-8, GM-CSF and GROα of cell supernatants was detected. The results demonstrated that the technology could characterize the living state by monitoring the extracellular water and investigate the hydration state inside a tumor cell in real time, showing great application potential for the label-free detection of normal cells and tumor cells of diverse malignant degree.
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