http://www.technologyreview.com/view/529926/terahertz-chip-identifies-short-strands-of-dna/
One of the more significant practical challenges currently occupying molecular biologists is to find better ways of identifying short strands of DNA. Called oligonucleotides, these strands of nucleotides are hugely useful in processes such as genetic testing, forensics and DNA amplification.
But identifying the strands is a somewhat laboured business. Almost every detection method relies on fluorescent dyes and markers that can be picked up by optical sensors providing a useful but indirect indication of the molecules that are present.
But molecular biologists would like a better
system that measures the characteristics of the molecules involved and so
provides direct evidence of the sequence of nucleotides. Indeed, various
research teams are working on such systems, some with significant success.
Today, Andrey Chernev at St
Petersburg Academic University in Russia and a few pals say they have
invented an entirely new way of identifying oligonucleotides using terahertz
radiation. “Our results demonstrate a new method for label-free, real-time
oligonucleotide characterisation,” they say.
An oligonucleotide is a short single-stranded DNA
or RNA molecule usually consisting of fewer than a hundred or so bases. The
sequence of these bases determines the type of oligonucleotide. So the ideal
detection mechanism would reveal this sequence.
Chernev and co’s idea is based on the way these molecules
resonate. They say that the sequence of bases in an oligonucleotide determines
the way in which the strand resonates at frequencies in the terahertz range.
Their idea is to capture a single oligonucleotide in a cavity filled with
terahertz waves that stimulates this resonant behaviour.
They begin by producing a signal as close as
possible to the resonant mode. By measuring the output from this cavity, they
can determine when the input spectra exactly matches the resonant modes of the
molecule. That tells them exactly what sort of oligonucleotide they have.
That’s the theory and they have tested it using a
device they call a silicon nanosandwich, a quantum well of p-type silicon
surrounded by barriers doped with boron. This produces terahertz radiation
inside the well where the oligonucleotide is deposited at a concentration that
allows a single molecule to enter.
They have tested the device on two
oligonucleotides, one that was 50 bases long and the other that was 100 bases
long and characterised their unique resonances. That allows them to distinguish
between the two oligonucleotides with relative ease at room temperature.
But this is just a first step. What is needed
next is an entire library of the unique signatures associated with every
oligonucleotide. That should be possible. Chernev and co aim to start by
analysing the resonances of each of the monomer and dimer molecules that make
up oligonucleotides. The signatures from these should provide a kind of
alphabet from which to work out the resonances of more complex polymers.
That is an interesting approach. But this is a
crowded area in which lots of good ideas are competing to become the standard
technique for identifying the molecules of life quickly and cheaply. Chernev
and co will have their work cut out to prove that their method is better than
the others that are emerging. But it certainly has potential and so is one to
watch for the future.
Ref: http://arxiv.org/abs/1407.6520 : DNA Detection By THz Pumping
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