Abstract-High-speed stimulated hyperspectral Raman imaging using rapid acousto-optic delay lines

Mohammed S. Alshaykh, Chien-Sheng Liao, Oscar E. Sandoval, Gregory Gitzinger, Nicolas Forget, Daniel E. Leaird, Ji-Xin Cheng, and Andrew M. Weiner

https://www.osapublishing.org/ol/abstract.cfm?uri=ol-42-8-1548&origin=search

Stimulated Raman scattering (SRS) is a powerful, label-free imaging technique that holds significant potential for medical imaging. To allow chemical specificity and minimize spectral distortion in the imaging of live species, a high-speed multiplex SRS imaging platform is needed. By combining a spectral focusing excitation technique with a rapid acousto-optic delay line, we demonstrate a hyperspectral SRS imaging platform capable of measuring a 3-dB spectral window of ∼200  cm−1$\sim 200{\mathrm{cm}}^{-1}$ within 12.8 μs with a scan rate of 30 KHz. We present hyperspectral images of a mixture of two different microsphere polymers as well as live fungal cells mixed with human blood.

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